Use the peak voltage and round up the spacing
Select the construction that matches the real board: internal conductors, uncoated external conductors, solder-masked conductors, conformally coated conductors or component leads. Then use the peak voltage across the gap.
The result is a minimum table value. Round up to the nearest practical manufacturing grid and check the applicable product safety standard for creepage, clearance and altitude requirements.
What this does not model
- Pollution degree, material group or comparative tracking index.
- Creepage across the board surface, slots or cutouts.
- Transient overvoltages, isolation barriers or regulatory testing.